Prolotherapy is a non-surgical injection procedure, typically using a sugar-water solution, that has the goal of relieving chronic pain. This therapy helps shorten and strengthen loose and damaged connective tissue (ligaments and tendons) that have not healed with rest or other non-surgical therapies. Prolotherapy is meant to promote a healing response in the damaged tissue, in turn easing pain and improving function. Prolotherapy is sometimes used to treat injured athletes. This procedure may also be called proliferative injection therapy or non-surgical ligament reconstruction.

Prolotherapy typically involves 15-20 injections to the affected soft tissue.